M. Cappello et al., ANCYLOSTOMA FACTOR XA INHIBITOR - PARTIAL-PURIFICATION AND ITS IDENTIFICATION AS A MAJOR HOOKWORM-DERIVED ANTICOAGULANT INVITRO, The Journal of infectious diseases, 167(6), 1993, pp. 1474-1477
Soluble protein extracts from adult Ancylostoma hookworms were found t
o contain an anticoagulant activity that markedly prolonged both the p
rothrombin time (PT) and partial thromboplastin time (PTT). By chromog
enic peptide substrate and clotting time assays, the anticoagulant act
ivity was attributed to a specific inhibitor of clotting factor Xa. Th
e hookworm anticoagulant was partially purified by ion-exchange column
chromatography. Those column fractions containing anti-Xa activity by
chromogenic assay also prolonged the PT and PTT as well as the factor
X (Stypven) clotting time. These data suggest that this potent factor
Xa inhibitor is a major anticoagulant from the adult Ancylostoma hook
worm.