ANCYLOSTOMA FACTOR XA INHIBITOR - PARTIAL-PURIFICATION AND ITS IDENTIFICATION AS A MAJOR HOOKWORM-DERIVED ANTICOAGULANT INVITRO

Citation
M. Cappello et al., ANCYLOSTOMA FACTOR XA INHIBITOR - PARTIAL-PURIFICATION AND ITS IDENTIFICATION AS A MAJOR HOOKWORM-DERIVED ANTICOAGULANT INVITRO, The Journal of infectious diseases, 167(6), 1993, pp. 1474-1477
Citations number
16
Categorie Soggetti
Infectious Diseases
ISSN journal
00221899
Volume
167
Issue
6
Year of publication
1993
Pages
1474 - 1477
Database
ISI
SICI code
0022-1899(1993)167:6<1474:AFXI-P>2.0.ZU;2-Y
Abstract
Soluble protein extracts from adult Ancylostoma hookworms were found t o contain an anticoagulant activity that markedly prolonged both the p rothrombin time (PT) and partial thromboplastin time (PTT). By chromog enic peptide substrate and clotting time assays, the anticoagulant act ivity was attributed to a specific inhibitor of clotting factor Xa. Th e hookworm anticoagulant was partially purified by ion-exchange column chromatography. Those column fractions containing anti-Xa activity by chromogenic assay also prolonged the PT and PTT as well as the factor X (Stypven) clotting time. These data suggest that this potent factor Xa inhibitor is a major anticoagulant from the adult Ancylostoma hook worm.