THE MOUSE RETINOID-X RECEPTOR-GAMMA GENE - GENOMIC ORGANIZATION AND EVIDENCE FOR FUNCTIONAL ISOFORMS

Retinoid-X receptors (RXRs) are a subfamily of the steroid/thyroid nuc lear receptor superfamily. There are three RXR genes: alpha, beta, and gamma, each with a distinct expression pattern and chromosomal locati on. In this study we describe the genomic organization of the mouse RX Rgamma gene, identify a second mouse (m) RXRgamma isoform, and map the exons of the two mRXRgamma isoforms on the gene. The new mRXRgamma is oform, called gamma2, has a unique 5'-untranslated region, and is expr essed highly in both cardiac and skeletal muscles, but very little in the liver, while the mRXRgamma1 is expressed in the brain and muscle. The gamma2 isoform is also a functional receptor and can transactivate the retinoid response elements of the apolipoprotein-AI and cellular retinol-binding protein-II genes. The mRXRgamma gene spans at least 50 kilobases of DNA on mouse chromosome 1 and has nine introns ranging f rom 107 basepairs to more than 30 kilobases. From the genomic separati on of the two unique 5'-ends of the mRXRgamma isoforms, it is suggeste d that this gene may have two promoters, similar to those described fo r the three RAR genes. The results of this study along with those of p reviously published studies suggest that there may be more than two is oforms of mRXRgamma.