TRANSIENT EXPRESSION OF THE BETA-GLUCURONIDASE GENE DELIVERED INTO UREDINIOSPORES OF UROMYCES-APPENDICULATUS BY PARTICLE BOMBARDMENT

Authors
LI AM ALTOSAAR I HEATH MC HORGEN PA
Citation
Am. Li et al., TRANSIENT EXPRESSION OF THE BETA-GLUCURONIDASE GENE DELIVERED INTO UREDINIOSPORES OF UROMYCES-APPENDICULATUS BY PARTICLE BOMBARDMENT, Canadian journal of plant pathology, 15(1), 1993, pp. 1-6
Citations number
NO
Categorie Soggetti
Plant Sciences
Journal title
Canadian journal of plant pathology → ACNP
ISSN journal
07060661
Volume
15
Issue
1
Year of publication
1993
Pages
1 - 6
Database
ISI
SICI code
0706-0661(1993)15:1<1:TEOTBG>2.0.ZU;2-0
Abstract
Transient expression of Escherichia coli beta-glucuronidase (GUS) gene is reported in Uromyces appendiculatus var. appendiculatus following bombardment of urediniospores with gold particles coated with plasmid DNA. The plasmid contained the chimaeric GUS gene driven by the caulif lower mosaic virus (CaMV) 35S promoter fragment. GUS activity could be detected both histochemically and spectrophotometrically after partic le introduction of the plasmid into the urediniospores.