DETECTION OF SIDEROPHORES IN GROWING CULTURES OF PSEUDOMONAS SPP

The siderophores produced by Pseudomonas fluorescens and P. chlororaph is were detected from the culture supernatants in MM9 and modified Kin g's medium by the universal CAS assay at wavelengths 620-690 nm. The C AS assay was applied to detect Pseudomonas siderophores directly in si tu, during their production phase, in modified King's medium. Optimum results were detected with a final CAS concentration of 0.025 mM and a n iron concentration of 1.25 muM. The problems of the method are discu ssed with respect to the absorbance spectrum, the toxicity of the HDTM A detergent, the influence of the iron concentration and the complexit y of media for siderophore production.