RAPID AND PERMANENT METHOD FOR TRIPLE IMMUNOPEROXIDASE STAINING IN RAT GASTRIC ANTRUM

A simple method of labeling 3 antigenic sites on 1 tissue section with only the conventional immunoperoxidase method is described. Although procedures to label 3 or more antigens simultaneously have been report ed previously, they have used combinations of fluorescence, immunopero xidase, and alkaline phosphatase to produce chromagens of different co lors. The triple-label immunoperoxidase procedure described is both co st- and time-effective, yields permanent results, and offers distinct advantages over other staining procedures.