FLOW-CYTOMETRIC ASSESSMENTS OF PLOIDY STABILITY IN CRYOPRESERVED DIHAPLOID SOLANUM-TUBEROSUM AND WILD SOLANUM SPECIES

Authors
WARD ACW BENSON EE BLACKHALL NW COOPERBLAND S POWELL W POWER JB DAVEY MR
Citation
Acw. Ward et al., FLOW-CYTOMETRIC ASSESSMENTS OF PLOIDY STABILITY IN CRYOPRESERVED DIHAPLOID SOLANUM-TUBEROSUM AND WILD SOLANUM SPECIES, Cryo-letters, 14(3), 1993, pp. 145-152
Citations number
29
Categorie Soggetti
Biology Miscellaneous",Physiology
Journal title
Cryo-letters → ACNP
ISSN journal
01432044
Volume
14
Issue
3
Year of publication
1993
Pages
145 - 152
Database
ISI
SICI code
0143-2044(1993)14:3<145:FAOPSI>2.0.ZU;2-N
Abstract
Ploidy stability in dihaploid cultivars of Solanum tuberosum and in wi ld genotypes, regenerated from cryopreserved shoot-tips, was assessed by flow cytometry. Frozen shoot-tips were compared with explants of po tato subjected to other in vitro manipulations. The frequency of polyp loidization was minimal in plants regenerated from cryopreservation as compared with those derived from leaf explants and protoplasts. Cryop reservation does not appear to induce ploidy changes in sensitive diha ploids of S. tuberosum.