SPATIAL AND TEMPORAL ACCUMULATION OF MESSENGER-RNAS ENCODING 2 COMMONLIGNIN PEROXIDASES IN PHANEROCHAETE-CHRYSOSPORIUM

Accumulation of peroxidases and their mRNAs was localized in colonies of Phanerochaete chrysosporium sandwiched between perforated polycarbo nate membranes. Northern (RNA) blot analyses of colonial rings and in situ hybridizations with specific probes for manganese(II)-dependent p eroxidase (MnP-1) and lignin peroxidase (LiP H8) mRNAs indicated that the expression of MnP-1 and Lip H8 genes started simultaneously in the central area of 3-day-old colonies. With time the signals for both tr anscripts spread to more-peripheral areas while decreasing in intensit y. Furthermore, the appearance of MnP protein, as detected with specif ic immune serum, immediately followed accumulation of the MnP-1 mRNA t ranscript. However, LiP protein could be detected only some time after accumulation of LiP H8 mRNA.