ISOLATION AND CHARACTERIZATION OF A GENE FROM TRYPANOSOMA-CRUZI ENCODING A 46-KILODALTON PROTEIN WITH HOMOLOGY TO HUMAN AND RAT TYROSINE AMINOTRANSFERASE

Authors
BONTEMPI EJ BUA J ASLUND L PORCEL B SEGURA EL HENRIKSSON J ORN A PETTERSSON U RUIZ AM
Citation
Ej. Bontempi et al., ISOLATION AND CHARACTERIZATION OF A GENE FROM TRYPANOSOMA-CRUZI ENCODING A 46-KILODALTON PROTEIN WITH HOMOLOGY TO HUMAN AND RAT TYROSINE AMINOTRANSFERASE, Molecular and biochemical parasitology, 59(2), 1993, pp. 253-262
Citations number
37
Categorie Soggetti
Parasitiology,Biology
Journal title
Molecular and biochemical parasitology → ACNP
ISSN journal
01666851
Volume
59
Issue
2
Year of publication
1993
Pages
253 - 262
Database
ISI
SICI code
0166-6851(1993)59:2<253:IACOAG>2.0.ZU;2-A
Abstract
The complete sequence of a gene encoding a 46-kDa protein of Trypanoso ma cruzi is presented. The first ATG complies with the consensus seque nce for initiation of translation. A single band of 2 kb was highlight ed by hybridizing a pro the 46-kDa protein gene to a Northern filter c ontaining total T. cruzi RNA. The gene is present in 50-80 copies per cell and most of them are contained in 2 tandem arrays on large T. cru zi chromosomes ( > 2000 kb). A strong homology with rat and human tyro sine aminotransferase was detected. Homology with a Trypanosoma brucei retrotransposon was found in the nonsense strand of the intergenic re gion.