T. Yoshikawa et al., ROLE OF ACTIVE OXYGEN, LIPID-PEROXIDATION, AND ANTIOXIDANTS IN THE PATHOGENESIS OF GASTRIC-MUCOSAL INJURY-INDUCED BY INDOMETHACIN IN RATS, Gut, 34(6), 1993, pp. 732-737
The roles of active oxygen, lipid peroxidation, and the antioxidative
defence mechanism in gastric mucosal injury induced by treatment with
indomethacin in rats were investigated. The total area of gastric eros
ions and concentration of lipid peroxides in the gastric mucosa increa
sed with time after administration of indomethacin (20 mg/kg, orally).
The alpha-tocopherol:total cholesterol ratio in serum was significant
ly decreased and the activity of glutathione peroxidase, an important
enzyme to scavenger of lipid peroxides, was inhibited by the administr
ation of indomethacin. Treatments with superoxide dismutase and catala
se inhibited the increases in gastric mucosal erosions and lipid perox
ides in the gastric mucosa, and the reduction of serum alpha-tocophero
l. Treatment with these scavengers did not improve the decreased gluta
thione peroxidase activity. These findings suggest that active oxygen
species and lipid peroxidation play an important part in the pathogene
sis of gastric mucosal injury induced by indomethacin, and that the de
creased glutathione peroxidase activity aggravated the injury due to a
ccelerated accumulation of hydrogen peroxide and lipid peroxides in th
e gastric mucosal cell.