Mg. Tigges et Rw. Loan, SERUM ANTIBODY-RESPONSE TO PURIFIED PASTEURELLA-HAEMOLYTICA CAPSULAR POLYSACCHARIDE IN CATTLE, American journal of veterinary research, 54(6), 1993, pp. 856-861
Capsular polysaccharide (CP) of Pasteurella haemolytica, biotype A, se
rotype 1, was purified and combined with saline solution, aluminum hyd
roxide, and Freund's incomplete (oil) adjuvant. Three groups of calves
were administered the various antigen preparations. The CP in saline
preparation was also administered to 5 mature cows. Second injections
were given 4 weeks after the first. Weekly obtained serum samples were
analyzed for P haemolyticalytica-specific antibody, using the indirec
t hemagglutination assay, and CP-specific antibodies were detected, us
ing an isotype-specific ELISA. Purified cp stimulated production of CP
-specific IgM, IgG1, and IgG2 in calves and predominantly IgM and IgG1
in mature cows. Significant increases in CP antibody titers were not
observed after the second injection of CP antigen in either calves or
mature cows. The CP in oil adjuvant stimulated the highest mean CP-spe
cific IgG1 and IgG2 responses, whereas the CP in aluminum hydroxide ad
juvant stimulated the highest mean CP-specific IgM response.