SELECTIVE HYDROLYSIS OF TRIGLYCERIDES WIT H SURFACTANT-COATED LIPASE

Several surfactant - coated enzymes were prepared by coating various o rigin of lipase with nonionic surfactant dioleylglutamic acid ester ri btol (2C18 DELTA9GE) whose structure is similar to that of lipid. Sele ctive hydrolysis reaction of triglycerides was investigated with the s urfactant - coated lipase in organic media under water content - limit ed conditions. In this reaction system, the most suitable solvent is i so octane and the surfactant - coated lipase prepared by Candida cylin dracea gives the highest hydrolysis yield of triolein. Selective hydro lysis of triolein can be performed by adjusting water content in iso o ctane. Assuming the Michaelis-Menten mechanism, apparent reaction rate constants were obtained by the analysis of initial reaction rate. The rate constant of surfactant - coated lipase was tens times that of po wder lipase.