R. Banerjee et Bc. Bhattacharyya, KINETIC-PROPERTIES OF EXTRACELLULAR ALKALINE PROTEASE OF RHIZOPUS-ORYZAE, Journal of fermentation and bioengineering, 75(5), 1993, pp. 380-382
Production of alkaline protease by Rhizopus species is an unusual phen
omenon. However, a locally isolated species of Rhizopus oryzae was fou
nd to secrete alkaline serine protease of industrial importance. The k
inetic parameter V (reaction velocity) of the purified fractionated en
zyme was evaluated under different environmental conditions and substr
ate to enzyme ratios. The Michaelis constant (K(m)) and maximum reacti
on velocity (V(max)) were also estimated.