EVIDENCE FOR HOMOLOGY OF BUFFALO AND GOAT GRANULAR CATIONIC PEPTIDES

Due to lack of studies on PMN granular cationic proteins to assess imm unostatus of buffaloes, the peripheral PMN leukocytes were subjected t o homogenization by sonication and centrifugation cycles in order to o btain pure pellet of granules. Acid extracted granules contained catio nic proteins and peptides, which were further separated by exclusion S ephadex G-50 chromatography, the peptides were in the last peak and we re found to be active against Gram-negative (Escherichia coli) and Gra m-positive (Staphylococcus aureus) bacteria. The peptides were conjuga ted to ovalbumin in order to develop antibodies, which were further te sted by Ouchterlony double immunodiffusion and enzyme linked immunosor bent assay. These tests demonstrated the antigenic relationship betwee n buffalo and goats for the PMN cationic peptides as well as the pepti de release which was also monitored in activated buffalo PMN leukocyte s.