MONOCLONAL-ANTIBODIES AGAINST HUMAN HEMOPEXIN AND THEIR APPLICATION

Six monoclonal antibodies raised against human serum hemopexin have be en characterized. The antibodies reacted with serum hemopexin as well as with the isolated protein in apo-form and with heme-protein complex es on immunoblots obtained following both PAGE and SDS-PAGE. In the ca se of PAGE blots 1 ng of hemopexin could be detected using a streptavi din-AP detection system. ELISA procedures employing two different pair s of monoclonal antibodies gave working ranges of 0.3-3 mg/l and 10-10 0 mug/l respectively.