Snh. Naqvi et al., TOXICITY OF NFD AGAINST SITOPHILUS-ORYZAE L EXPOSED TO IMPREGNATED FILTER-PAPER AND ITS EFFECT ON PHOSPHATASES AND PROTEIN METABOLITES, Acta Biologica Cracoviensia. Series Zoologia, 33, 1992, pp. 49-58
Toxicity of NfD i.e. neutral fraction of winter neem leaves containing
nimocinolide and isonimocinolide as major components (tetranor-triter
penoids), extracted from Azadirachta indica was tested againt one week
old rice weevils Sitophilus oryzae L. Its effect on enzyme activity (
acid phophatase and alkaline phosphatase) was determined as well as th
e effect on protein metabolites was also observed by TLC method. The L
D50 was calculated to be 0.0392 mg/sq.cm with the help of graph drawn
on the log-log graph paper. Inhibition of alkaline phosphatase was 13.
17% and acid phosphate 4.28% by NfD. Inhibition of protein was also ob
served by TLC.