GROWTH-INHIBITION BY TRANSFORMING GROWTH FACTOR-BETA(TGF-BETA) TYPE-IIS RESTORED IN TGF-BETA-RESISTANT HEPATOMA-CELLS AFTER EXPRESSION OF TGF-BETA RECEPTOR TYPE-II CDNA

Authors
INAGAKI M MOUSTAKAS A LIN HY LODISH HF CARR BI
Citation
M. Inagaki et al., GROWTH-INHIBITION BY TRANSFORMING GROWTH FACTOR-BETA(TGF-BETA) TYPE-IIS RESTORED IN TGF-BETA-RESISTANT HEPATOMA-CELLS AFTER EXPRESSION OF TGF-BETA RECEPTOR TYPE-II CDNA, Proceedings of the National Academy of Sciences of the United Statesof America, 90(11), 1993, pp. 5359-5363
Citations number
33
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Proceedings of the National Academy of Sciences of the United Statesof America → ACNP
ISSN journal
00278424
Volume
90
Issue
11
Year of publication
1993
Pages
5359 - 5363
Database
ISI
SICI code
0027-8424(1993)90:11<5359:GBTGFT>2.0.ZU;2-P
Abstract
The growth of human hepatoma Hep 3B cells is potently inhibited by TGF -beta1 (ID50 = 0.2 ng/ml, 8 pM). A mutant cell line was derived that w as not inhibited in growth by TGF-beta1 at 5 ng/ml (200 pM) and that l acked TGF-beta receptor type II (TGF-betaRII) gene. Transfection of th e cloned cDNA for human TGF-betaRII to this mutant cell line restored receptor expression as well as the inhibition in growth by TGF-beta1. In both wild-type and mutant cells stably transfected with TGF-betaRII cDNA, TGF-betaRII coimmunoprecipitated with TGF-beta receptor type I in the presence of ligand. These experiments provide direct evidence f or the role of TGF-betaRII in the inhibitory effect of TGF-beta on gro wth and suggest that TGF-betaRII acts by means of a heteromeric surfac e complex with TGF-beta receptor type I.