Fl. Smith et Wl. Dewey, ENDOGENOUS DYNORPHIN MODULATES CALCIUM-MEDIATED ANTINOCICEPTION IN MICE, Pharmacology, biochemistry and behavior, 45(2), 1993, pp. 383-391
We previously reported that calcium administered IT produces antinocic
eption by stimulating spinal Met-enkephalin release. However, at times
the antinociceptive effects of calcium in the tail-flick test are gre
atly diminished. The results of this study indicates that during these
periods calcium also stimulates endogenous dynorphin release. Dynorph
in has been reported to block opiate-induced antinociception. Calcium-
injected mice (150-600 nmol, IT) pretreated with vehicle IP displayed
a poor degree of antinociception. Alternatively, pretreating mice with
pentobarbital (45 mg/kg, IP) restored the antinociceptive effects of
calcium. Low doses of naloxone and norbinaltorphimine (BNI) did not pr
oduce antinociception but restored the antinociceptive effects of calc
ium. Dynorphin (1-17) (Dyn 1-17), and Dyn (1-13), but not Dyn (1-8), b
locked the antinociceptive effects of calcium restored with pentobarbi
tal. These results indicate that calcium-mediated antinociception was
sensitive to injected dynorphins. In additional experiments, antiserum
to Dyn (1-13) was found to restore the antinociceptive effects of cal
cium, presumably by binding dynorphin released by calcium.