Mg. Rambotti et al., ULTRACYTOCHEMICAL LOCALIZATION OF PARTICULATE GUANYLATE-CYCLASE IN RAT ADRENAL-GLAND EXPOSED TO STIMULATION BY PORCINE BRAIN NATRIURETIC PEPTIDE, Histochemical Journal, 25(5), 1993, pp. 384-391
We studied the cytochemical localization of particulate guanylate cycl
ase (GC) in rat adrenal gland after stimulation with porcine brain nat
riuretic peptide (pBNP) by electron microscopy. In the adrenal cortex,
GC activity, as demonstrated by the presence of reaction product, was
prevalently localized to the zona glomerulosa and zona fasciculata, w
hile the zona reticularis showed little GC reaction product. In the ad
renal medulla, GC reaction product was present only in adrenalin-conta
ining cells. All GC positivity was associated with intracellular membr
anes. No GC reaction product was detected in specimens incubated in me
dia devoid of pBNP. In parallel samples incubated in the presence of r
at atrial natriuretic factor (rANF), the distribution of rANF-stimulat
ed GC activity was similar to that of pBNP-stimulated GC activity.