Je. Stern et al., INVOLVEMENT OF THE CERVICAL SYMPATHETIC NERVOUS-SYSTEM IN THE CHANGESOF CALCIUM HOMEOSTASIS DURING TURPENTINE OIL-INDUCED STRESS IN RATS, Neuroendocrinology, 57(3), 1993, pp. 381-387
Hypocalcemia is a common finding during stress. The objective of this
study was to examine: (a) the changes in circulating calcium, parathyr
oid hormone (PTH) and calcitonin (CT) concentration in rats stressed b
y being given a subcutaneous injection of turpentine oil, and (b) the
involvement of the sympathetic cervical pathway in stress-induced chan
ges of calcium homeostasis. Four hours after receiving turpentine oil
or vehicle, rats were subjected either to hypocalcemia, by being given
EDTA intraperitoneally, or to hypercalcemia, by being injected CaCl2
intraperitoneally. Significant changes in serum calcium (10% decrease)
, serum PTH (28% increase) and CT levels (40% decrease) were observed
in stressed rats. EDTA administration brought about a significantly gr
eater hypocalcemia, and a higher PTH secretory response in turpentine
oil-stressed rats. During stress, the increase of serum calcium after
CaCl2 was significantly smaller, and the rise of CT was greater than i
n controls. In the case of CT the changes were still observed in rats
subjected to superior cervical ganglionectomy (SCGx) 14 days earlier.
In the case of PTH, the increase found in stressed rats, but not the a
ugmented response after EDTA, was blunted by SCGx. The potentiation of
hypocalcemia brought about by turpentine oil was no longer observed i
n SCGx rats. In vehicle-treated controls, SCGx delayed PTH response to
hypocalcemia, but did not affect the increased response of CT to CaCl
2 challenge. The results indicate that a number of changes in calcium
homeostasis arise during turpentine oil stress in rats. SCGx was effec
tive to modify the set point for PTH release, but played a minor role
in affecting the augmentation of CT release during stress.