Jr. Phillips et al., BIOLOGICAL PRODUCTION OF ETHANOL FROM COAL SYNTHESIS GAS - MEDIUM DEVELOPMENT STUDIES, Applied biochemistry and biotechnology, 39, 1993, pp. 559-571
The anaerobic bacteria Clostridium ljungdahlii produces ethanol and ac
etate from CO, CO2, and H-2 in synthesis gas. Early studies with the b
acterium showed that relatively high concentrations of ethanol could b
e produced by lowering the fermentation pH and eliminating yeast extra
ct from the medium in favor of a defined medium. This article presents
the results from a medium development study based on the aerobic bact
erium Escherichia coli. The results of continuous-reactor studies in a
continuously stirred tank reactor (CSTR) with and without cell recycl
e are shown to demonstrate the utility of this improved medium.