Pseudomonas putida, capable of utilizing acetonitrile as a sole source
of carbon and nitrogen, was isolated from contaminated soil and water
samples collected from industrial sites. The P. putida cells were imm
obilized in calcium alginate beads. The degradation of acetonitrile by
the immobilized cells of P. putida was investigated. The immobilized
cells degraded different concentrations of acetonitrile into ammonia a
nd carbon dioxide. The effect of aeration on the degradation rate was
also studied. Oxygen limitation was suggested in the alginate-immobili
zed system. The rate of degradation of acetonitrile increased with inc
rease in the rate of aeration.