A SULFIDE-LINKED OLIGONUCLEOTIDE ANALOG WITH SELECTIVE HYBRIDIZATION PROPERTIES

Authors
MENG B KAWAI SH WANG DG JUST G GIANNARIS PA DAMHA MJ
Citation
B. Meng et al., A SULFIDE-LINKED OLIGONUCLEOTIDE ANALOG WITH SELECTIVE HYBRIDIZATION PROPERTIES, Angewandte Chemie, International Edition in English, 32(5), 1993, pp. 729-731
Citations number
22
Categorie Soggetti
Chemistry
Journal title
Angewandte Chemie, International Edition in English → ACNP
ISSN journal
05700833
Volume
32
Issue
5
Year of publication
1993
Pages
729 - 731
Database
ISI
SICI code
0570-0833(1993)32:5<729:ASOAWS>2.0.ZU;2-8
Abstract
RNA and DNA sequences can be distinguished efficiently with a modified oligonucleotide incorporating nonhydrolyzable sulfide linkages. A dod ecamer containing three thio-ether-linked riboT-T dimers 1 is easily p repared by standard solid-phase methods and binds cooperatively to com plementary RNA but not to DNA. Oligonucleotides of this type should be of use in antisense technology.