DETERMINATION OF ANTI-ASPERGILLUS ACTIVITY OF ANTIFUNGAL AGENTS BASEDON THE DYNAMIC GROWTH-RATE OF A SINGLE HYPHA

The dynamic growth rate of a single hypha of Aspergillus niger was ana lysed using an automatic system. A colony of A. niger was in contact w ith saline, saline containing an antifungal agent, and flushing saline , in sequence. The growth rate of a test hypha selected arbitrarily fr om the colony responded dynamically to the antifungal agent. The minim um concentration that caused the complete inhibition of hyphal growth was defined as the minimum inhibitory concentration (MIC). The MIC val ues obtained were compared with those determined by conventional metho ds based on increasing rate of colony diameter or dry matter weight.