LOCALIZATION OF XANTHINE DEHYDROGENASE MESSENGER-RNA IN HORSE SKELETAL-MUSCLE BY IN-SITU HYBRIDIZATION WITH DIGOXIGENIN-LABELED PROBE

In situ hybridization was used to localize xanthine dehydrogenase (XDH ) mRNA in horse skeletal muscle. Capillary endothelial cells were foun d to express XDH, but muscle cells did not give any signal. The digoxi genin-labelled probe was produced by PCR with primers based on the cDN A sequence of mouse XDH and horse lung cDNAs. A 4.3 kb mRNA was detect ed in a Northern blot.