STRUCTURAL-ANALYSIS OF OLIGOSACCHARIDE BISPHOSPHATES ISOLATED FROM THE LIPOPOLYSACCHARIDE OF A RECOMBINANT STRAIN OF ESCHERICHIA-COLI F515 (RE CHEMOTYPE) EXPRESSING THE GENUS-SPECIFIC EPITOPE OF CHLAMYDIA LIPOPOLYSACCHARIDE

Authors
HOLST O BROER W THOMASOATES JE MAMAT U BRADE H
Citation
O. Holst et al., STRUCTURAL-ANALYSIS OF OLIGOSACCHARIDE BISPHOSPHATES ISOLATED FROM THE LIPOPOLYSACCHARIDE OF A RECOMBINANT STRAIN OF ESCHERICHIA-COLI F515 (RE CHEMOTYPE) EXPRESSING THE GENUS-SPECIFIC EPITOPE OF CHLAMYDIA LIPOPOLYSACCHARIDE, European journal of biochemistry, 214(3), 1993, pp. 703-710
Citations number
46
Categorie Soggetti
Biology
Journal title
European journal of biochemistry → ACNP
ISSN journal
00142956
Volume
214
Issue
3
Year of publication
1993
Pages
703 - 710
Database
ISI
SICI code
0014-2956(1993)214:3<703:SOOBIF>2.0.ZU;2-K
Abstract
The lipopolysaccharide of the recombinant strain Escherichia coli F515 -207, expressing the genus-specific epitope of Chlamydia lipopolysacch aride, was sequentially de-O- and de-N-acylated by mild hydrazinolysis and treatment with 4 M KOH, respectively, yielding two oligosaccharid e bisphosphates which were isolated by high-performance anion-exchange chromatography and gel-permeation chromatography. Their structures we re determined by chemical analysis, NMR spectroscopy, and mass spectro metry as -4)-alpha-Kdo-(2-6)-beta-D-GlcN-(1-6)-alpha-D-GlcN 1,4'-P, (t etrasaccharide bisphosphate) and )-alpha-Kdo-(2-4)-alpha-Kdo-(2-6)-bet a-D-GlcN-(1-6 )-alpha-D-GlcN 1,4'-P2 (pentasaccharide bisphosphate).