PHENOTYPIC AND FUNCTIONAL-CHARACTERIZATION OF A NEW HUMAN MACROPHAGE CELL-LINE K1M DEMONSTRATING IMMUNOPHAGOCYTIC ACTIVITY AND SIGNALING THROUGH HLA CLASS-II
Dp. Dialynas et al., PHENOTYPIC AND FUNCTIONAL-CHARACTERIZATION OF A NEW HUMAN MACROPHAGE CELL-LINE K1M DEMONSTRATING IMMUNOPHAGOCYTIC ACTIVITY AND SIGNALING THROUGH HLA CLASS-II, Immunology, 90(4), 1997, pp. 470-476
A human macrophage line, designated Klm, has been established from per
ipheral blood. Klm expresses a number of lineage-specific markers as w
ell as a broad array of intercellular adhesion molecules. In particula
r, Klm expresses high levels of human leucocyte antigen (HLA) class I
and class II. In response to ligation of HLA class II (HLA-DR), but no
t in response to ligation of HLA class I, Klm forms tighter homotypic
aggregates and develops a striking 'stellate' culture phenotype. Klm a
lso expresses Fc receptors for immunoglobulin G (IgG) (CD64, CD32, and
CD16) and can be shown to phagocytose polystyrene latex beads, as wel
l as neuroblastoma cells in the presence of tumour-specific monoclonal
antibody (mAb). The Klm cell line should therefore prove useful for s
tudying both signalling through macrophage HLA class II and immunophag
ocytosis.