DELETIONS ON THE LONG ARM OF CHROMOSOME-17 IN PILOCYTIC ASTROCYTOMA

Pilocytic astrocytomas are the most common astrocytic tumors of childh ood and differ clinically and histopathologically from those astrocyto mas that affect adults. Studies of adult astrocytic tumors have reveal ed allelic losses on chromosomes 10, 17p, 19q and alterations in the e pidermal growth factor receptor (EGFR) gene. We have previously examin ed pilocytic astrocytomas for allelic losses on chromosomes 10 and 19q and for amplification of the EGFR gene, but did not detect genomic al terations at these loci. In the present study we assayed 20 pilocytic astrocytomas for loss of allelic heterozygosity of chromosome 17p, inc luding one locus in the p53 tumor suppressor gene. In addition. becaus e pilocytic astrocytomas frequently affect patients with neurofibromat osis type 1 (NF1) and the NF1 gene has been mapped to 17q11.2, we also examined multiple loci on the long arm of chromosome 17. Allelic loss was observed on chromosome 17 in four cases (three sporadic, one NF1) ; all lost portions of the long arm in chromosome 17, and one tumor lo st the short arm as well. One tumor showed an interstitial deletion on the long arm that included the region of the NF1 gene. These data sug gest the presence of a tumor suppressor gene on 17q that is associated with pilocytic astrocytomas. A potentiel candidate for this gene is t he NF1 tumor suppressor gene.