STUDIES IN CAPILLARY ZONE ELECTROPHORESIS WITH A POSTCOLUMN MULTIPLE CAPILLARY DEVICE FOR FRACTION COLLECTION AND STEPWISE INCREASE IN ELECTROOSMOTIC FLOW DURING ANALYSIS
W. Nashabeh et al., STUDIES IN CAPILLARY ZONE ELECTROPHORESIS WITH A POSTCOLUMN MULTIPLE CAPILLARY DEVICE FOR FRACTION COLLECTION AND STEPWISE INCREASE IN ELECTROOSMOTIC FLOW DURING ANALYSIS, Electrophoresis, 14(5-6), 1993, pp. 407-416
A new approach involving the stepwise increase in electroosmotic flow
during analysis in capillary zone electrophoresis is introduced and ev
aluated in the rapid separations of proteins and peptides. The stepwis
e increase in electroosmotic flow is based on the principle of coupled
capillaries in series having different flow characteristics, a concep
t that was introduced recently by our laboratory. To produce stepwise
changes in electroosmotic flow during analysis, a post-column multiple
capillary device, which allowed the switching between several coupled
capillary systems, was designed and constructed in-house. The utility
of the multiple capillary device was also demonstrated and extended t
o fraction collection of separated analytes in short capillary segment
s, The fraction collection in capillaries facilitated the quantitative
transfer of the collected fractions to high performance liquid chroma
tography (HPLC) for further analysis or to mass spectrometry (MS) for
structural determination. The off-line combination of capillary zone e
lectrophoresis with HPLC or MS utilized commercial instruments without
the need of expensive interfacing designs.