APPLICATIONS OF THE MONOCLONAL-ANTIBODY PC10 ASSESSMENT OF PROLIFERATIVE GRADE IN CELL SMEARS

In view of the increasing need to assess the proliferative activity of hematologic malignancies, slide-based methods to quantify the prolife rating cell nuclear antigen (PCNA) were developed and evaluated. Two t echniques to evaluate this antigen were adapted to the infiltration le vel of the disease. The first one is particularly appropriate to massi ve invasion and is based on the alkaline phosphatase-antialkaline phos phatase complex (APAAP)method. The second one is reserved for reduced infiltration and uses a double immunofluorescence labeling. One is spe cific for the target cell to be identified and the other one is specif ic for the PCNA. These techniques permit an easy and accurate routine evaluation of cell cycle marker expression in hematologic malignancies .