EVALUATION OF THE L-PYRROLIDONYL-BETA-NAPHTHYLAMIDE HYDROLYSIS TEST FOR THE DIFFERENTIATION OF MEMBERS OF THE FAMILIES ENTEROBACTERIACEAE AND VIBRIONACEAE

Authors
CHAGLA AH BORCZYK AA ALDOM JE ROSA SD COLE DD
Citation
Ah. Chagla et al., EVALUATION OF THE L-PYRROLIDONYL-BETA-NAPHTHYLAMIDE HYDROLYSIS TEST FOR THE DIFFERENTIATION OF MEMBERS OF THE FAMILIES ENTEROBACTERIACEAE AND VIBRIONACEAE, Journal of clinical microbiology, 31(7), 1993, pp. 1946-1948
Citations number
11
Categorie Soggetti
Microbiology
Journal title
Journal of clinical microbiology → ACNP
ISSN journal
00951137
Volume
31
Issue
7
Year of publication
1993
Pages
1946 - 1948
Database
ISI
SICI code
0095-1137(1993)31:7<1946:EOTLHT>2.0.ZU;2-T
Abstract
A simple, rapid, and inexpensive spot test incorporating the substrate pyrrolidonyl naphthylamide was used to examine pyrrolidonyl peptidase activity among 800 bacterial strains belonging to the families Entero bacteriaceae and Vibrionaceae. The pyrrolidonyl naphthylamide test was found to be particularly useful in separating Citrobacter spp. (100% positive) from Salmonella spp. (0.4% positive) and Escherichia coli (0 % positive). Furthermore, it would appear to offer a safer alternative to the traditional potassium cyanide test for differentiating citroba cters from salmonellae.