Ft. Delbeke et M. Debackere, INFLUENCE OF HYDROLYSIS PROCEDURES ON THE URINARY CONCENTRATIONS OF CODEINE AND MORPHINE IN RELATION TO DOPING ANALYSIS, Journal of pharmaceutical and biomedical analysis, 11(4-5), 1993, pp. 339-343
A method is described for the GC-NPD determination of urinary codeine
and morphine after derivatization with trifluoroacetic anhydride. The
lower limit for accurate quantitative determination was 0.05 mug ml-1.
After the oral administration of Bisolvon Griblettes(R) corresponding
to 30 mg codeine phosphate to seven subjects maximum codeine concentr
ations were obtained after 1-2 h and codeine remained detectable gener
ally 24 h post dosing. The mean maximum level was 5.1 +/- 2.8 mug ml-1
found after enzymatic hydrolysis with Suc Helix pomatia juice (SHP).
Based on these and previous results (mean 6.3 +/- 3.4 mug ml-1) a thre
shold level for codeine of 16 mug ml-1 is proposed. Significant differ
ences were noticed between urinary codeine concentrations found after
enzymatic hydrolysis with SHP, beta-glucuronidase from Patella vulgata
and acid hydrolysis, respectively. Generally, highest values were obt
ained after SHP, while beta-glucuronidase and especially acid hydrolys
is resulted in much lower levels. No morphine could be detected after
acid hydrolysis. Concerning doping analysis, in particular the uniform
ity of methods and interpretation of the results, it is recommended th
at the hydrolysis method should be specified in the rules of those spo
rting federations allowing codeine and/or morphine.