MEASUREMENT OF ASCORBIC-ACID IN HUMAN AQUEOUS-HUMOR AND PLASMA AND BOVINE AQUEOUS-HUMOR BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION

Authors
WATSON DG IQBAL Z MIDGLEY JM PRYCEJONES H MORRISON L DUTTON GN KARDITSAS S WILSON W
Citation
Dg. Watson et al., MEASUREMENT OF ASCORBIC-ACID IN HUMAN AQUEOUS-HUMOR AND PLASMA AND BOVINE AQUEOUS-HUMOR BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH ELECTROCHEMICAL DETECTION, Journal of pharmaceutical and biomedical analysis, 11(4-5), 1993, pp. 389-392
Citations number
19
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
Journal of pharmaceutical and biomedical analysis → ACNP
ISSN journal
07317085
Volume
11
Issue
4-5
Year of publication
1993
Pages
389 - 392
Database
ISI
SICI code
0731-7085(1993)11:4-5<389:MOAIHA>2.0.ZU;2-O
Abstract
High-performance liquid chromatography (HPLC) on ODS silica with cetyl trimethylammonium bromide as an ion-pairing reagent and electrochemica l detection (ECD) was used to determine ascorbic acid in bovine and hu man aqueous humour and human plasma. Hydroquinone was used as the inte rnal standard. A calibration curve plotted with ascorbic acid concentr ations in the range 0.5-5 mug ml-1 for peak height versus internal sta ndard peak height had a correlation coefficient of 0.998. The RSD (pre cision) between analyses of the same diluted sample was 1.5% and the R SD (reproducibility) between analyses of separate aliquots of the same sample of aqueous humour was 1.6%.