DETERMINATION OF ATROPINE SULFATE AND BENZALKONIUM CHLORIDE IN EYE DROPS BY HPLC

Authors
SANTONI G TONSINI A GRATTERI P MURA P FURLANETTO S PINZAUTI S
Citation
G. Santoni et al., DETERMINATION OF ATROPINE SULFATE AND BENZALKONIUM CHLORIDE IN EYE DROPS BY HPLC, International journal of pharmaceutics, 93(1-3), 1993, pp. 239-243
Citations number
12
Categorie Soggetti
Pharmacology & Pharmacy
Journal title
International journal of pharmaceutics → ACNP
ISSN journal
03785173
Volume
93
Issue
1-3
Year of publication
1993
Pages
239 - 243
Database
ISI
SICI code
0378-5173(1993)93:1-3<239:DOASAB>2.0.ZU;2-#
Abstract
A reversed-phase high-performance liquid chromatographic method has be en developed for the simultaneous determination of atropine sulphate a nd C-12-C-16 benzalkonium chloride homologues in eye drops. The elutio n was isocratic using a C-8 column and acetonitrile-diluted acetic aci d (80:20, v/v) as mobile phase with 6 mM tetramethylammonium bromide. Detection was carried out at 260 nm with a diode array. Tropic acid ca n also be detected and quantified under the same chromatographic condi tions.