EFFECT OF HIGH ULTRALOW DOSES OF ALPHA-TO COPHEROL ON THE KINETIC-PARAMETERS OF PROTEIN-KINASE-C

Authors
MALTSEVA YL KURNAKOVA NV PALMINA NP BURLAKOVA YB
Citation
Yl. Maltseva et al., EFFECT OF HIGH ULTRALOW DOSES OF ALPHA-TO COPHEROL ON THE KINETIC-PARAMETERS OF PROTEIN-KINASE-C, Biologiceskie membrany, 9(10-11), 1992, pp. 1028-1030
Citations number
10
Categorie Soggetti
Cytology & Histology
Journal title
Biologiceskie membrany → ACNP
ISSN journal
02334755
Volume
9
Issue
10-11
Year of publication
1992
Pages
1028 - 1030
Database
ISI
SICI code
0233-4755(1992)9:10-11<1028:EOHUDO>2.0.ZU;2-5
Abstract
We studied the dependence of protein kinase C substrate phosphorylatio n on histone H-1 at the action of alpha-tocopherol as inhibitor at two concentrations (10(-4) M and 10(-14) M). The dependence was found to be bell-form both in the presence and absence of alpha-tocopherol. The positive kinetic cooperativity (Hill coefficient n(H) = 1.9 +/- 0.2) was observed for the ultralow alpha-tocopherol concentration but not f or the high one. The affinity of protein kinase C to histone H-1 was t wo times higher in experiments with 10(-14) M of alpha-tocopherol than using 10(-4) M.