STABLE DECREASE IN ATP CONTENTS IN CULTUR ED CEREBELLAR AND HIPPOCAMPAL-NEURONS DURING AND AFTER GLUTAMATE TOXIC TREATMENT

Authors
BOGACHEV AV BYKOVA LP KHODOROV BI ANDREEVA NA KHASPEKOV LG PINELIS VG VICTOROV IV
Citation
Av. Bogachev et al., STABLE DECREASE IN ATP CONTENTS IN CULTUR ED CEREBELLAR AND HIPPOCAMPAL-NEURONS DURING AND AFTER GLUTAMATE TOXIC TREATMENT, Biologiceskie membrany, 9(10-11), 1992, pp. 1057-1059
Citations number
7
Categorie Soggetti
Cytology & Histology
Journal title
Biologiceskie membrany → ACNP
ISSN journal
02334755
Volume
9
Issue
10-11
Year of publication
1992
Pages
1057 - 1059
Database
ISI
SICI code
0233-4755(1992)9:10-11<1057:SDIACI>2.0.ZU;2-K
Abstract
Changes in ATP contents in cultured rat cerebellar granule cells and m ouse hippocampal neurons during and after glutamate (Glu) treatment we re measured using the luciferase-luciferin method. After a 15-min Glu treatment (50 muM, 0 Mg2+), the ATP contents (in nmol/ng of protein) d ecreased approximately twofold, and remained at this or even lower lev el after a 430-min washing of cultures by control solution. This resul t is of importance for understanding the origin of Glu-induced destabi lization of Ca2+ homeostasis, since both Ca2+-extruding systems, Na(o) +/Ca(i)2+ exchange and Ca2+ pump, are known to be ATP - dependent.