Dc. Kong et al., GENE-4-HELICASE OF BACTERIOPHAGE-T7 MEDIATES STRAND TRANSFER THROUGH PYRIMIDINE DIMERS, MISMATCHES, AND NONHOMOLOGOUS REGIONS, Proceedings of the National Academy of Sciences of the United Statesof America, 94(7), 1997, pp. 2987-2992
In bacteriophage T7 the gene 2.5 single-stranded DNA-binding protein a
nd the gene 4 helicase together promote the annealing of homologous re
gions of two DNA partners to form a joint molecule and subsequent stra
nd transfer, In this reaction T7 gene 2.5 protein is essential for joi
nt molecule formation, but is not required for T7 gene 4 protein-media
ted strand transfer, T7 gene 4 helicase alone is able to mediate stran
d transfer, provided that a joint molecule is available, The present p
aper shows that, in addition, strand transfer proceeds at a normal rat
e even when both DNA partners contain ultraviolet-induced pyrimidine d
imers (0.6 dimer per 100 nt), An insert of a relatively long (842-nt)
segment of nonhomologous DNA in the single-stranded DNA partner has no
effect on strand transfer, whereas its presence in the double-strande
d partner prevents strand transfer, A short insert (37 nt) can be tole
rated in either partner, Thus, DNA helicase is able to participate in
recombinational DNA repair through its role in strand exchange, provid
ing a pathway distinct from nucleotide excision repair.