INHIBITION OF CI857-CONTROLLED RECOMBINANT GENE-EXPRESSION IN ESCHERICHIA-COLI AT VERY-LOW CONCENTRATIONS OF GLUCOSE

The effects of glucose on biomass and temperature-mediated recombinant gene expression has been studied in Escherichia coli batch cultures. Glucose, above 2 g/L, has inhibitory effects on aerobic cell growth an d specially on gene expression. Although lower concentrations have no influences on biomass, a tenuous. but still remarkable concentration-d ependent reduction of gene expression levels was observed even at abou t 0.1 g/L.