PROPERTIES OF A BACTERIOCIN PRODUCED BY CARNOBACTERIUM-PISCICOLA CP5

Carnobacterium piscicola CP5 produced a bacteriocin named carnocin CP5 that inhibited Carnohacterium, Enterococcus and Listeria spp. and amo ng the Lactobacillus spp. only Lactobacillus delbrueckii ssp.. Carnoci n CP5 was stable 1 h at 100-degrees-C at pH 7.0. It was inactivated by numerous proteolytic enzymes. Production of carnocin, CP5 occured in MRS broth regulated at pH 7.0. The apparent molecular weight of the ba cteriocin in the crude extract was greater than 10 kDa, but around 5 k Da after action of SDS or urea. Novobiocin treatment led to non-produc er variants.