PEPTIDE-SYNTHESIS CATALYZED BY CYNARA-CARDUNCULUS L ACID PROTEASE IN AQUEOUS-ORGANIC BIPHASIC SYSTEMS

Authors
BARROS MT FARO CJ PIRES EMV
Citation
Mt. Barros et al., PEPTIDE-SYNTHESIS CATALYZED BY CYNARA-CARDUNCULUS L ACID PROTEASE IN AQUEOUS-ORGANIC BIPHASIC SYSTEMS, Biotechnology letters, 15(6), 1993, pp. 653-656
Citations number
7
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
15
Issue
6
Year of publication
1993
Pages
653 - 656
Database
ISI
SICI code
0141-5492(1993)15:6<653:PCBCLA>2.0.ZU;2-N
Abstract
The ability of Cynara cardunculus L. protease to perform peptide synth esis was investigated using an aqueous organic biphasic system and the amino acid derivatives CBZ.Phe and Met.OMe. The reaction products wer e separated by RP-HPLC and identified by amino acid analysis and by EI -MS. Significant amounts of the dipeptide CBZ.Phe.Met.OMe was produced within 24h by Cynara cardunculus L. protease as compared with the amo unt produced by pepsin under the same conditions.