We have determined the complete nucleotide sequence of a 23 kb segment
from the left arm of chromosome IV, which is carried by the cosmid 1L
10. This sequence contains the 3' coding region of the STE7 and RET1 (
COP1) genes, and 13 complete open reading frames longer than 300 bp, o
f which ten correspond to putative new genes and three (CLB3, MSH5 and
RPC53) have been sequenced previously. The sequence from cosmid 1L10
was obtained entirely by a combined subcloning and walking primer stra
tegy.