CHARACTERIZATION AND CHEMOSENSITIVITY OF A HUMAN EPITHELIOID SARCOMA CELL-LINE (SARCCR-2)

A new cell line was derived from the epithelioid sarcoma of a Caucasia n woman who had previously received chemotherapy. The cells grew as an adherent monolayer, with a doubling time of 28 hr and had mainly epit helial morphology, but with areas of mesenchymal-like cytoplasmic exte nsions. The cells were tumorigenic in nude mice, with a short growth t ime, and a doubling time of 8 days. The cell line showed over-expressi on of P-glycoprotein by Western blot analysis, and its sensitivity to doxorubicin and vincristine was low. This sensitivity could be enhance d by reversants of multidrug resistance (MDR), such as cyclosporin or verapamil. This cell line constitutes an excellent model for studying compounds able to reverse MDR. (C) 1993 Wiley-Liss, Inc.