RRR-ALPHA-TOCOPHERYL SUCCINATE INHIBITS PROLIFERATION AND ENHANCES SECRETION OF TRANSFORMING GROWTH-FACTOR-BETA (TGF-BETA) BY HUMAN BREAST-CANCER CELLS

Authors
CHARPENTIER A GROVES S SIMMONSMENCHACA M TURLEY J ZHAO BH SANDERS BG KLINE K
Citation
A. Charpentier et al., RRR-ALPHA-TOCOPHERYL SUCCINATE INHIBITS PROLIFERATION AND ENHANCES SECRETION OF TRANSFORMING GROWTH-FACTOR-BETA (TGF-BETA) BY HUMAN BREAST-CANCER CELLS, Nutrition and cancer, 19(3), 1993, pp. 225-239
Citations number
31
Categorie Soggetti
Nutrition & Dietetics",Oncology
Journal title
Nutrition and cancer → ACNP
ISSN journal
01635581
Volume
19
Issue
3
Year of publication
1993
Pages
225 - 239
Database
ISI
SICI code
0163-5581(1993)19:3<225:RSIPAE>2.0.ZU;2-5
Abstract
The RRR-alpha-tocopheryl succinate form of vitamin E inhibits the prol iferation of estrogen receptor-positive and estrogen receptor-negative human breast cancer cell lines in a dose-dependent manner in vitro. A nalyses of cell-conditioned medium from RRR-alpha-tocopheryl succinate growth-inhibited cells revealed the presence of a potent antiprolifer ative activity. Characterization of the antiproliferative activity as transforming growth factor-beta (TGF-beta) was established by 1) growt h inhibition of the TGF-beta-responsive Mv1Lu-CCL-64 mink lung and mur ine CTLL-2 cell lines, 2) combination of physical characteristics incl uding heat stability, acid stability, and Bio-Gel P-60 column chromato graphy elution profile, and 3) neutralization of the antiproliferative activity in the conditioned media by antibodies specific for TGF-beta .