IMPROVED LYSIS OF WINE LACTOBACILLI FOR HIGH-YIELD ISOLATION AND CHARACTERIZATION OF CHROMOSOMAL DNA

Authors
VELAZQUEZ JB CANSADO J SIEIRO C CALO P LONGO E VILLA TG
Citation
Jb. Velazquez et al., IMPROVED LYSIS OF WINE LACTOBACILLI FOR HIGH-YIELD ISOLATION AND CHARACTERIZATION OF CHROMOSOMAL DNA, Journal of microbiological methods, 17(4), 1993, pp. 247-253
Citations number
19
Categorie Soggetti
Microbiology,"Biochemical Research Methods
Journal title
Journal of microbiological methods → ACNP
ISSN journal
01677012
Volume
17
Issue
4
Year of publication
1993
Pages
247 - 253
Database
ISI
SICI code
0167-7012(1993)17:4<247:ILOWLF>2.0.ZU;2-V
Abstract
An improved lysis of Lactobacillus casei and Lactobacillus fermentum w ine strains with respect to alternative protocols is described. This l ysis protocol yielded 600 to 800 mug of high molecular weight DNA (25- 40 kb) per liter of culture. Characterization of the molecular size of Sau3A-restricted DNA from L. casei, packaging into pBR322 and stable maintenance in E. coli were achieved. This lysis procedure may be an a lternative method for the isolation of high molecular weight DNA from lysis-resistant microorganisms from different environmental sources.