NUCLEIC-ACID HYBRIDIZATION ON NITROCELLULOSE FILTERS OF VACUUM SPOTTED ESCHERICHIA-COLI BACTERIAL-CELLS

Authors
IVANOV I DRAGULEV B ABOUHAIDAR MG
Citation
I. Ivanov et al., NUCLEIC-ACID HYBRIDIZATION ON NITROCELLULOSE FILTERS OF VACUUM SPOTTED ESCHERICHIA-COLI BACTERIAL-CELLS, Journal of microbiological methods, 17(4), 1993, pp. 305-310
Citations number
7
Categorie Soggetti
Microbiology,"Biochemical Research Methods
Journal title
Journal of microbiological methods → ACNP
ISSN journal
01677012
Volume
17
Issue
4
Year of publication
1993
Pages
305 - 310
Database
ISI
SICI code
0167-7012(1993)17:4<305:NHONFO>2.0.ZU;2-K
Abstract
The in situ techniques for DNA and RNA colony hybridization were adapt ed for application to Escherichia coli bacterial cells grown in liquid media and loaded onto membrane filters by microfiltration. A linear c orrelation was found between the amount of loaded cells and the P-32-r adioactivity retained on the filters. This makes it possible to apply both techniques for quantitative determination of specific polynucleot ides produced at different stages of bacterial growth.