APPLICATION OF ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE SEROEPIZOOTIOLOGICAL SURVEY OF ANTIBODIES AGAINST PORCINE CYTOMEGALOVIRUS

Authors
TAJIMA T HIRONAO T KAJIKAWA T KAWAMURA H
Citation
T. Tajima et al., APPLICATION OF ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE SEROEPIZOOTIOLOGICAL SURVEY OF ANTIBODIES AGAINST PORCINE CYTOMEGALOVIRUS, Journal of veterinary medical science, 55(3), 1993, pp. 421-424
Citations number
13
Categorie Soggetti
Veterinary Sciences
Journal title
Journal of veterinary medical science → ACNP
ISSN journal
09167250
Volume
55
Issue
3
Year of publication
1993
Pages
421 - 424
Database
ISI
SICI code
0916-7250(1993)55:3<421:AOEFTS>2.0.ZU;2-H
Abstract
Enzyme-linked immunosorbent assay (ELISA) for the detection of antibod ies against porcine cytomegalovirus (PCMV) was developed using the OF- 1 strain of PCMV as an antigen. Results of the ELISA were compared to those of indirect fluorescent antibody (IFA) and serum neutralization (SN) tests. ELISA and IFA test were found to be extremely sensitive mo re than SN test. All of 11 tested sera were highly reactive in both EL ISA and IFA test, but 6 of them were antibody-negative in the SN test. The retrospective survery of 436 fattening pig sera collected in Japa n in 1981 showed that 433 (99.4%) of them were highly antibody-positiv e in ELISA.