Z. Yu et Sr. Ikeda, 2,3-BUTANEDIONE MONOXIME BLOCKADE OF CA2-RAT SYMPATHETIC NEURONS DOESNOT INVOLVE CHEMICAL PHOSPHATASE-ACTIVITY( CURRENTS IN ADULT), Neuroscience letters, 155(1), 1993, pp. 24-28
Effects of the 'chemical phosphatase' 2,3-butanedione monoxide (BDM) o
n voltage-activated Ca2+ currents of adult rat superior cervical gangl
ion neurons were investigated using the whole-cell patch-clamp techniq
ue. BDM produced a rapid (<10 s), reversible and dose-dependent (IC50
= 18.3 mM) inhibition of Ca2+ currents. The action of BDM was not prev
ented by 1 mM 8-(4-chlorophenylthio)-cAMP or 50 muM isoproterenol. H-7
, a non-specific protein kinase inhibitor at 200 muM, did not prevent
the rapid recovery from BDM-induced inhibition. Our results suggest th
at BDM inhibition of Ca2+ currents in rat sympathetic neurons does not
require a 'chemical phosphatase' activity.