RETENTION CHARACTERISTICS OF A BETA-CYCLODEXTRIN POLYMER-COATED LIQUID-CHROMATOGRAPHIC COLUMN

A silica based, beta-cyclodextrin polymer-coated liquid chromatographi c column was prepared and its retention characteristics were determine d using 28 ring-substituted phenol derivatives as solutes and methanol -water mixtures as eluents. The column showed a good separation power; the log k' value decreased linearly with increasing methanol concentr ation. Stepwise regression analysis demonstrated that the lipophilicit y does not determine the retention on the beta-cyclodextrin polymer. A ccordingly, the retention mechanism deviates from that of traditional reversed-phase columns. The steric characteristics and to a lesser ext ent the electronic parameters of the solutes have the greatest impact on the retention, which means that the inclusion complex formation als o prevails after polymerization, resulting in a modified retention beh aviour of the complexed solutes.