KARYOTYPE AND FISH ANALYSIS OF A NEWLY ESTABLISHED CELL-LINE DERIVED FROM A HUMAN BLADDER-CARCINOMA

A new human malignant urologic cell line was established in vitro from a moderately differentiated transitional cell carcinoma of the bladde r and cytogenetically characterized. Repeated chromosome analyses of t he cell line using conventional RHG and GTG; banding and non-radioacti ve in situ hybridization showed a stable karyotype with a modal number of 48 and chromosomal rearrangements, some of which have not been pre viously described. Numerical deviation included three trisomies (+7, 8, +9) and one nullisomy (-19, -19). Structural changes involved a bal anced translocation (1;5)(q12;q12), an isochromosome 3q, a 14p+, and t wo markers. Fluorescence in situ hybridization (FISH), using biotin-la beled alpha satellite probes for chromosome 9 or painting for chromoso mes 1 and 8, applied to interphase nuclei or metaphases showed similar results to those found by conventional cytogenetic study. This cell l ine may be an interesting model for fuller characterization by molecul ar biology studies and for testing anti-cancer drugs in vitro.