PERSISTENT SPECTRAL HOLE-BURNING OF NATIVE SYNECHOCOCCUS ANTENNA COMPLEXES

Persistent spectral holes were burned into fluorescence spectra of per ipheral and core antenna complexes of Synechococcus elongatus cells an d thylakoid membranes at 4.2 K. The observed hole structure with antih ole and phonon side-band hole is typical for non-photochemical mechani sm. The zero-phonon holes are suggested to be broadened by fast energy transfer. The comparison of peripheral and core antenna hole burned s pectra is discussed together with the effect of the polyacrylamide gel isolation procedure of the core complex. The results obtained on the antenna complexes are compared with an in vitro reference system lacki ng energy transfer.