STUDY ON PREIRRADATION GRAFTING OF BSA NASI CONJUGATES ON AGAROSE ANDITS APPLICATION

In this work we used N-acryloxysuccinimide(NASI) with a function group as the intermediate to graft the Bovine Serum Albumin(BSA), passthrou gh BSA / NAST conjugates, onto agarose which has been pre-irradiated i n Co-60 gamma-source. Preparation of BSA / NASI conjugates was carried out at 37-degrees-C for 2h,the molar ratio of BSA and NASI in reactio n is 1:6.Pre-irradiation of agarose sample was carried out at room tem perature in air and grafting was performed below 37-degrees-C for 5h u nder bubbling nitrogen.The total dose used was lower than 7 kGy. This technique is applicable to immobilize BSA and other proteins or separa te the tryptophans enantiomer as well.