M. Yi et al., STUDY ON PREIRRADATION GRAFTING OF BSA NASI CONJUGATES ON AGAROSE ANDITS APPLICATION, Radiation physics and chemistry, 42(4-6), 1993, pp. 927-930
Citations number
4
Categorie Soggetti
Nuclear Sciences & Tecnology","Chemistry Physical","Physics, Atomic, Molecular & Chemical
In this work we used N-acryloxysuccinimide(NASI) with a function group
as the intermediate to graft the Bovine Serum Albumin(BSA), passthrou
gh BSA / NAST conjugates, onto agarose which has been pre-irradiated i
n Co-60 gamma-source. Preparation of BSA / NASI conjugates was carried
out at 37-degrees-C for 2h,the molar ratio of BSA and NASI in reactio
n is 1:6.Pre-irradiation of agarose sample was carried out at room tem
perature in air and grafting was performed below 37-degrees-C for 5h u
nder bubbling nitrogen.The total dose used was lower than 7 kGy. This
technique is applicable to immobilize BSA and other proteins or separa
te the tryptophans enantiomer as well.